Estimating the Nature of Leishmania Major Different Immune Reactions in Laboratory Model Animals After Alum-Alm Injection

Abstract

Introduction & Objective: This study is to determine how to react the vertebrate immune system after receiving or injecting Autoclaved leishmania Major with Alum-AluM and the main propose of conducting this study is that the immunity in leishmaniasis is often cellular, so it is expected that Vaccine-induced immunity formed by the predominance of cellular immunity.
Materials and Methods: In this regard some statistical review and number of research samples in 5 groups is considered. Each group included 5 BALB/c mice and each group consisted of two replicates.
Results: Titer challenges with Alum-Alm vaccine (i.e. new injected) with 3 booster dosage that equal to P >1:100 which means the test is negative. Titer challenge with Alum-Alm Vaccine (new injected) with 4 boosters that equal with P >1:100. Titer from mice vaccinated (i.e. past month after last injection that was sampled) with 3 boosters (P >1:100). The titer challenge with Alum vaccine with 4 boosters (after past month of their injection) P > 1:100. The titer for group 1 of vaccinated with two boosters as (P>1:100).
Conclusion: Based on this study it was revealed that the immunity orientation after injection of the same doses of Alum-ALM in BALB/c mice progressed to TH1 and IFN-Y stimulation and not seen any meaningful differences in IL-4 ELISA test (color reaction against 1: 400 dilution) and Alum-ALM injection failed to stimulate the TH2 response thus the results was as (P>1:100) so it was considered as negative test.